104-00-228 PolyAn 2D-Epoxy glass sheets - 110 x 74 x 1 mm (5 sheets/box)

2D-Epoxy Glass Sheets (110x74x1mm), 5 sheets/box

The 2D-epoxy glass sheets are manufactured from quality substrate with high flatness surface smoothness. The glass has very low background fluorescence. 

These glass sheets are compatible with most microarray printers and slide scanners / imagers. We recommend the axiREADERs for both colorimetric and fluorometric applications.

Surface Modification

The glass sheets are coated with a very thin epoxy silane layer that will covalently bind most biomolecules, including oligonucleotides, proteins, peptides and small molecules.

Epoxides are cyclic ethers with a highly strained three-member ring. Epoxy rings can be easily reacted with nucleophiles e.g. amines, hydrazines, thiols, hydroxides and carboxyl groups. Compared to NHS-esters or 1,4-Phenylene isothiocyates (PDITC) the epoxy surface is more stable and has a longer shelf-life.

Epoxy surfaces are stable up to temperatures of 40° C and are also more stable against humidity compared to NHS and PDITC-surfaces.

The nucleophilic addition is catalysed by acid or basic conditions. Under acidic conditions, the oxygen in the ring is positively charged, which facilitates the nucleophilic attack. Under basic conditions the least substituted carbon is attacked by the applied nucleophile in a standard SN2 reaction.

The 2D-epoxy glass sheets are also availabler in larger quantities upon request.