104-00-208 PolyAn 3D-Epoxy glass sheets - 110 x 74 x 1 mm (5 sheets/box)

3D-Epoxy Glass Sheets, 110 x 74 x 1 mm, 5 sheet/box

The 3D-epoxy glass sheets are manufactured from quality substrate with high flatness surface smoothness. The glass has very low background fluorescence. 

These slides are compatible with all microarray printers and slide scanners / imagers. We recommend the axiREADERs for both colorimetric and fluorometric applications.

Surface Modification

PolyAn’s 3D-functionalised microarray slides are functionalized with a three-dimensional (3D)-surface chemistry comprised of a long-chain polymer containing a defined number of reactive groups. This polymer is covalently linked to the surface of the glass surface. 

PolyAn 3D-epoxy glass surface's reactive groups consists of epoxides. The epoxides will covalently bind most biomolecules, including oligonucleotides, proteins, peptides and small molecules.

Epoxides are cyclic ethers with a highly strained three-member ring. Epoxy rings can be easily reacted with nucleophiles e.g. amines, hydrazines, thiols, hydroxides and carboxyl groups. Compared to NHS-esters or 1,4-Phenylene isothiocyates (PDITC) the epoxy surface is more stable and has a longer shelf-life.

Epoxy surfaces are stable up to temperatures of 40° C and are also more stable against humidity compared to NHS and PDITC-surfaces.

The nucleophilic addition is catalyzed by acid or basic conditions. Under acidic conditions, the oxygen in the ring is positively charged, which facilitates the nucleophilic attack. Under basic conditions the least substituted carbon is attacked by the applied nucleophile in a standard SN2 reaction.

Multipart Plates - bottom sheet 

PolyAn’s multipart plates are comprised of a functionalized glass sheet which is combined with a well superstructure after the printing process. This product is for the glass sheet only.

Key features of multipart plates:

• Fast printing on planar surface
• No electrostatic effects

Technical specifications

Dimensions: The functionalized glass sheets are produced with standard dimensions of 75 x 110 x 1 mm.

Packaging: The sheets are packaged in inert atmospheric boxes.

Storage: PolyAn’s epoxy slides and plates are characterized by a long shelf-life when stored dry, at 4-8°C and protected from sunlight. All slides are packaged in boxes under Argon atmosphere to avoid contamination with particles. The Argon atmosphere also minimizes degradation of the reactive surface through contact with air or humidity.