00-705-301 PolyAn 3D-Aldehyde 96-well multipart plate - glass bottom - square wells

3D-Aldehyde Glass Bottom Plate (96-well, square, 2/box)

PolyAn 3D-Aldehyde glass bottom plate are manufactured with high substrate flatness and surface smoothness to ensure a uniform hybridization layer. They are usually used for covalent coupling of Amine-containing (bio)molecules.

The glass has low background fluorescence to enhance signal detection and improve data quality. They are suitable for both low-density and high-density microarray applications, including those used in high-throughput screening.

These glass bottom 'slides' are compatible with all microarray printers and some scanners / imagers. We recommend the axiREADERs for both colorimetric and fluorometric applications. The glass bottom can be imaged before attaching the well superstructure for QC purposes.

Surface Modification

PolyAn’s 3D-functionalised microarray slides are functionalized with a three-dimensional (3D)-surface chemistry comprised of a long-chain polymer containing a defined number of reactive groups. This polymer is covalently linked to the surface of the glass surface. 

PolyAn 3D-aldehyde glass surface's reactive groups consists of aldehyde groups, designed to form covalent bonds with primary amines found on most biomolecules.

Aldehyde groups react with amines, hydrazines, and aminoalkoxyacetyl-modified biomolecules, initially forming unstable imines (Schiff bases). These imines can be stabilized by reduction with NaBH4 or NaCNBH3 to yield stable amines. After coupling, unreacted aldehydes should be blocked using small molecules that penetrate the 3D matrix and quench remaining reactive sites.

Aldehyde slides are often used for amino-linked DNA probe immobilization in applications like gene expression analysis, genotyping, and other genomic studies.

Multipart Plates

PolyAn’s multipart plates are comprised of a functionalized glass plate which is combined with a well superstructure after the printing process.

Key features of multipart plates:

• Fast printing on planar surface
• No electrostatic effects

Technical specifications

Dimensions: The functionalized glass plate are produced with standard dimensions of 75 x 110 x 1 mm.

Packaging: The slides are packaged in inert atmospheric boxes.

Storage: PolyAn’s aldehyde slides and plates are characterized by a long shelf-life when stored dry, at 4-8°C and protected from sunlight. All slides are packaged in boxes under Argon atmosphere to avoid contamination with particles. The Argon atmosphere also minimizes degradation of the reactive surface through contact with air or humidity.